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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- MHCD1401 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (TuK4), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- TuK4
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Development of a skin- and neuro-attenuated live vaccine for varicella.
Mesenchymal stem cells negatively regulate CD4(+) T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.
Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells-A Pilot Study.
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Alterations in the properties of the cell membrane due to glycosphingolipid accumulation in a model of Gaucher disease.
CD14 and IL18 gene polymorphisms associated with colorectal cancer subsite risks among atomic bomb survivors.
Regulation of osteoarthritis-associated key mediators by TNFα and IL-10: effects of IL-10 overexpression in human synovial fibroblasts and a synovial cell line.
Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors.
Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.
Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.
Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.
Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.
Wang W, Pan D, Fu W, Ye X, Han J, Yang L, Jia J, Liu J, Zhu R, Zhang Y, Liu C, Ye J, Selariu A, Que Y, Zhao Q, Wu T, Li Y, Zhang J, Cheng T, Zhu H, Xia N
Nature communications 2022 Feb 11;13(1):824
Nature communications 2022 Feb 11;13(1):824
Mesenchymal stem cells negatively regulate CD4(+) T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.
Gong B, Zheng L, Lu Z, Huang J, Pu J, Pan S, Zhang M, Liu J, Tang J
Molecular medicine reports 2021 Jan;23(1)
Molecular medicine reports 2021 Jan;23(1)
Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells-A Pilot Study.
Herten M, Zilkens C, Thorey F, Tassemeier T, Lensing-Höhn S, Fischer JC, Sager M, Krauspe R, Jäger M
Molecules (Basel, Switzerland) 2019 Jan 15;24(2)
Molecules (Basel, Switzerland) 2019 Jan 15;24(2)
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Cassetta L, Fragkogianni S, Sims AH, Swierczak A, Forrester LM, Zhang H, Soong DYH, Cotechini T, Anur P, Lin EY, Fidanza A, Lopez-Yrigoyen M, Millar MR, Urman A, Ai Z, Spellman PT, Hwang ES, Dixon JM, Wiechmann L, Coussens LM, Smith HO, Pollard JW
Cancer cell 2019 Apr 15;35(4):588-602.e10
Cancer cell 2019 Apr 15;35(4):588-602.e10
Alterations in the properties of the cell membrane due to glycosphingolipid accumulation in a model of Gaucher disease.
Batta G, Soltész L, Kovács T, Bozó T, Mészár Z, Kellermayer M, Szöllősi J, Nagy P
Scientific reports 2018 Jan 9;8(1):157
Scientific reports 2018 Jan 9;8(1):157
CD14 and IL18 gene polymorphisms associated with colorectal cancer subsite risks among atomic bomb survivors.
Hu Y, Yoshida K, Cologne JB, Maki M, Morishita Y, Sasaki K, Hayashi I, Ohishi W, Hida A, Kyoizumi S, Kusunoki Y, Tokunaga K, Nakachi K, Hayashi T
Human genome variation 2015;2:15035
Human genome variation 2015;2:15035
Regulation of osteoarthritis-associated key mediators by TNFα and IL-10: effects of IL-10 overexpression in human synovial fibroblasts and a synovial cell line.
Mrosewski I, Jork N, Gorte K, Conrad C, Wiegand E, Kohl B, Ertel W, John T, Oberholzer A, Kaps C, Schulze-Tanzil G
Cell and tissue research 2014 Jul;357(1):207-23
Cell and tissue research 2014 Jul;357(1):207-23
Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.
Asin SN, Fanger MW, Wildt-Perinic D, Ware PL, Wira CR, Howell AL
The Journal of infectious diseases 2004 Jul 15;190(2):236-45
The Journal of infectious diseases 2004 Jul 15;190(2):236-45
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74
Journal of virology 2003 Jul;77(13):7563-74
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES
Journal of virology 2003 Jul;77(13):7563-74
Journal of virology 2003 Jul;77(13):7563-74
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8
Cytometry. Part B, Clinical cytometry 2003 Jul;54(1):10-8
Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors.
Slobedman B, Mocarski ES, Arvin AM, Mellins ED, Abendroth A
Blood 2002 Oct 15;100(8):2867-73
Blood 2002 Oct 15;100(8):2867-73
Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.
Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11
Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.
Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11
Clinical and diagnostic laboratory immunology 2002 Mar;9(2):403-11
Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.
Giavedoni LD, Velasquillo MC, Parodi LM, Hubbard GB, Hodara VL
Journal of virology 2000 Feb;74(4):1648-57
Journal of virology 2000 Feb;74(4):1648-57
Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.
Giavedoni LD, Velasquillo MC, Parodi LM, Hubbard GB, Hodara VL
Journal of virology 2000 Feb;74(4):1648-57
Journal of virology 2000 Feb;74(4):1648-57
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood monocytes stained using FITC of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Association of mCD14 ( a ), sCD14 ( b ) and plasma IL-18 ( c ) levels with CD14 and IL18 gene polymorphisms in non-exposed, cancer-free subjects. Log-transformed (base 10) protein levels were analyzed using the analysis of variance method. MFI, mean of fluorescence intensity.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 v7D induces functional activation of human DCs in vitro. Human iDCs were generated from CD14 + monocytes purified from PBMCs, and were treated with rOka, v7D, vOka (MOI = 0.01) or mock-infected MRC-5 cell lysate as a control. a , b Representative flow cytometry data and summary of VZV-gE expression on iDCs at 3 dpi. rOka-infected iDCs were stained with an isotype antibody (iso) as a control. Numbers on the right side of each graph in ( a ) represent the percent of VZV-gE-positive cells. c Flow cytometry analysis of expression of the cell-surface costimulatory markers CD40, CD80, CD83 and CD86 on DCs at 3 dpi. Representative flow cytometry data are shown in Supplementary Fig. 5 . d Analysis of percent lysis of DCs compared to that of MRC-5 cells after inoculation with v7D and vOka (MOI = 0.01) over five days. e Cytokine/chemokine analysis of DC culture supernatants. f Analysis of cell proliferation rates of the purified autologous CD4 + and CD8 + T cells after co-culture with antigen-pulsed DCs using the BrdU ELISA assay. g , h Representative flow cytometry data and summary data of CFSE dilution among CD4 + and CD8 + T cells after 5-day co-culture with antigen-pulsed DCs. i Analysis of IFN-gamma production in CD4 + and CD8 + T cells after 5 days of co-culture with antigen-pulsed DCs using an ELISPOT assay. The results are represented as averages +- the SD ( n = 3 per group). Asterisks denote a significant difference ( P < 0.05) compared to the mock controls as determine
- Conjugate
- Green dye
