Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
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Validation data
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- Product number
- MHCD1404-4 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (TuK4), PE
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- TuK4
- Vial size
- 2 mL
- Storage
- 4° C, store in dark
Submitted references miR-181a Modulation of ERK-MAPK Signaling Sustains DC-SIGN Expression and Limits Activation of Monocyte-Derived Dendritic Cells.
Physical exercise and human adipose-derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease.
Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.
Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.
Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.
Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver.
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
Lim CX, Lee B, Geiger O, Passegger C, Beitzinger M, Romberger J, Stracke A, Högenauer C, Stift A, Stoiber H, Poidinger M, Zebisch A, Meister G, Williams A, Flavell RA, Henao-Mejia J, Strobl H
Cell reports 2020 Mar 17;30(11):3793-3805.e5
Cell reports 2020 Mar 17;30(11):3793-3805.e5
Physical exercise and human adipose-derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease.
Cucarián JD, Berrío JP, Rodrigues C, Zancan M, Wink MR, de Oliveira A
Journal of neuroscience research 2019 Sep;97(9):1095-1109
Journal of neuroscience research 2019 Sep;97(9):1095-1109
Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.
Onzi GR, Ledur PF, Hainzenreder LD, Bertoni AP, Silva AO, Lenz G, Wink MR
Cytotherapy 2016 Jul;18(7):828-37
Cytotherapy 2016 Jul;18(7):828-37
Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.
Weiskopf K, Anderson KL, Ito D, Schnorr PJ, Tomiyasu H, Ring AM, Bloink K, Efe J, Rue S, Lowery D, Barkal A, Prohaska S, McKenna KM, Cornax I, O'Brien TD, O'Sullivan MG, Weissman IL, Modiano JF
Cancer immunology research 2016 Dec;4(12):1072-1087
Cancer immunology research 2016 Dec;4(12):1072-1087
Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.
Bacchetta J, Chun RF, Gales B, Zaritsky JJ, Leroy S, Wesseling-Perry K, Boregaard N, Rastogi A, Salusky IB, Hewison M
PloS one 2014;9(12):e116530
PloS one 2014;9(12):e116530
Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver.
Fomin ME, Tai LK, Bárcena A, Muench MO
Stem cells and development 2011 Jul;20(7):1247-57
Stem cells and development 2011 Jul;20(7):1247-57
The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.
Elsner L, Flügge PF, Lozano J, Muppala V, Eiz-Vesper B, Demiroglu SY, Malzahn D, Herrmann T, Brunner E, Bickeböller H, Multhoff G, Walter L, Dressel R
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002
Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.
Strickland LA, Ross J, Williams S, Ross S, Romero M, Spencer S, Erickson R, Sutcliffe J, Verbeke C, Polakis P, van Bruggen N, Koeppen H
The Journal of pathology 2009 Jul;218(3):380-90
The Journal of pathology 2009 Jul;218(3):380-90
Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.
Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, Felici C, Falcieri E, Reilly JT, Barnett D, Papa S
Journal of immunological methods 2004 Dec;295(1-2):67-78
Journal of immunological methods 2004 Dec;295(1-2):67-78
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood monocytes stained using R-PE of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Morphological features and characterization of ADSCs. Our mesenchymal stromal cells had a spindle-shaped, fibroblast-like morphology, and demonstrated plastic adherent properties in culture. Cells underwent differentiation in (a) chondrocytes stained by Alcian blue; (c) Adipocytes showing some lipid vesicles stained by Oil red; and (e) osteoblasts, showing calcium phosphate stained with Alizarin red, after 3 weeks of induction. The negative controls, which received only the standard culture medium at the same time as those induced are showing for comparison (b,d,f). (g) Histograms of flow cytometry showing that these cells were homogeneously positive for mesenchymal markers: CD44, and CD105; but negative for hematopoietic markers: CD14, CD34, and CD45. Microphotograph scale = 50 mum [Color figure can be viewed at wileyonlinelibrary.com ]
- Conjugate
- Yellow dye