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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6955 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6955, RRID:AB_1572280
- Product name
- CD14 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of CD14.
- Antigen sequence
C-KRVDADADPRQYAD- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CD14 polyclonal antibody (Cat # PAB6955) (1 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CD14 polyclonal antibody (Cat # PAB6955) (4 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
