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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Protein array [1]
- Other assay [2]
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- Product number
- V3758 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- LPSR/2385
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- CD14 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2385). Predicted molecular weight: 40-55 kDa depending on glycosylation level.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human ThP-1 cell lysate with CD14 antibody (clone LPSR/2385). Predicted molecular weight: 40-55 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lymph node with CD14 antibody (clone LPSR/2385). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with CD14 antibody (clone LPSR/2385). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lymph node with CD14 antibody (clone LPSR/2385). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with CD14 antibody (clone LPSR/2385). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with CD14 antibody (clone LPSR/2385); Red=isotype control, Blue= CD14 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD14 antibody (clone LPSR/2385). These results demonstrate the foremost specificity of the LPSR/2385 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD14 antibody (clone LPSR/2385) as confirmation of integrity and purity.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD14 antibody (clone LPSR/2385) as confirmation of integrity and purity.
