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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- H007T03Y01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Yellow 570, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MEM-15 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.
- Antibody clone number
- MEM-15
- Concentration
- 4 µL/Test
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NovaFluor Yellow 570 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NovaFluor Yellow 570 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were either left unstained (blue histogram) or stained with CD14 Monoclonal Antibody, NovaFluor Yellow 570 (Product # H007T03Y01) (purple histogram) and acquired in the YG1 channel on a 5-laser Cytek Aurora. Cells in the monocyte gate were used in the analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD14 Monoclonal Antibody, NovaFluor Yellow 570 (Product # H007T03Y01) (right). Data was acquired in the YG1 channel on a 5-laser Cytek Aurora and singlet cells were used for analysis.
