500-P320-100UG

antibody from Invitrogen Antibodies
Targeting: CD14
Provider product page for 500-P320-100UG
Western blot
ELISA

Antibody data

Product number
500-P320-100UG - Provider product page
Provider
Invitrogen Antibodies
Product name
CD14 (soluble) Polyclonal Antibody, PeproTech®
Antibody type
Polyclonal
Antigen
Recombinant full-length protein
Description
AA Sequence of recombinant protein: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human sCD14-specific antibody was purified by affinity chromatography employing an immobilized Human sCD14 matrix. Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sCD14 (500-P320Bt) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD14. Western Blot: To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity
Human
Host
Rabbit
Vial size
100 μg
Concentration
0.1-1.0 mg/mL
Storage
-20°C
CD14 protein structure - 500-P320-100UG shown in red.