Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-86166 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86166, RRID:AB_11025390
- Product name
- Rabbit Polyclonal CD14 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CD14 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer Patients.
Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas.
Kobayashi M, Chung JS, Beg M, Arriaga Y, Verma U, Courtney K, Mansour J, Haley B, Khan S, Horiuchi Y, Ramani V, Harker D, Gopal P, Araghizadeh F, Cruz PD Jr, Ariizumi K
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jan 15;25(2):828-838
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jan 15;25(2):828-838
Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
Bachmann J, Burté F, Pramana S, Conte I, Brown BJ, Orimadegun AE, Ajetunmobi WA, Afolabi NK, Akinkunmi F, Omokhodion S, Akinbami FO, Shokunbi WA, Kampf C, Pawitan Y, Uhlén M, Sodeinde O, Schwenk JM, Wahlgren M, Fernandez-Reyes D, Nilsson P
PLoS pathogens 2014 Apr;10(4):e1004038
PLoS pathogens 2014 Apr;10(4):e1004038
Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas.
Wu CC, Hsu CW, Chen CD, Yu CJ, Chang KP, Tai DI, Liu HP, Su WH, Chang YS, Yu JS
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1100-17
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1100-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody [NBP1-86166] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody [NBP1-86166] - Analysis using Anti-CD14 antibody NBP1-86166 (A) shows similar pattern to independent antibody NBP1-86168 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Analysis in human lymph node and pancreas tissues. Corresponding CD14 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody NBP1-86166 (A) shows similar protein distribution across tissues to independent antibody NBP1-86168 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human tonsil shows moderate membranous positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NBP1-86166] - Staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.