Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2807 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2807, RRID:AB_10002272
- Product name
- Goat Polyclonal CD14 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo.
The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.
Martin I, Cabán-Hernández K, Figueroa-Santiago O, Espino AM
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3924-36
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3924-36
The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.
Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H
Infection and immunity 2001 Jun;69(6):3772-81
Infection and immunity 2001 Jun;69(6):3772-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD14 Antibody [NB100-2807] - Staining of PBL lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD14 Antibody [NB100-2807] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. (4 ug/ml)