HPA001887

antibody from Atlas Antibodies

Targeting: CD14

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA001887

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001887, RRID:AB_1078442

Product name

Anti-CD14

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVE
IHAGGLNLEPFLKRVDADADPRQYADTVKALRVRR
LTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKI
TGTMPPLPLEATGLALSSL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Increased CTLA-4(+) T cells and an increased ratio of monocytes with loss of class II (CD14(+) HLA-DR(lo/neg)) found in aggressive pediatric sarcoma patients.

Hingorani P, Maas ML, Gustafson MP, Dickman P, Adams RH, Watanabe M, Eshun F, Williams J, Seidel MJ, Dietz AB
Journal for immunotherapy of cancer 2015;3:35

---

LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14.

Tarassishin L, Suh HS, Lee SC
Glia 2014 Jun;62(6):999-1013

---

Functional contribution of elevated circulating and hepatic non-classical CD14CD16 monocytes to inflammation and human liver fibrosis.

Zimmermann HW, Seidler S, Nattermann J, Gassler N, Hellerbrand C, Zernecke A, Tischendorf JJ, Luedde T, Weiskirchen R, Trautwein C, Tacke F
PloS one 2010 Jun 10;5(6):e11049

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-CD14 antibody HPA001887 (A) shows similar pattern to independent antibody HPA002127 (B).

Antibody #2 product nr

HPA002127

Antibody provider

Atlas Antibodies

Show more

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line SiHa shows localization to vesicles.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human lymph node and pancreas tissues using HPA001887 antibody. Corresponding CD14 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human liver, lymph node, pancreas and rectum using Anti-CD14 antibody HPA001887 (A) shows similar protein distribution across tissues to independent antibody HPA002127 (B).

Antibody #2 product nr

HPA002127

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows cytoplasmic positivity in immune cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows moderate to strong cytoplasmic positivity in lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows moderate to strong membranous positivity in hepatic sinusoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate membranous positivity in germinal center cells.

Sample type

HUMAN