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- Immunohistochemistry [5]
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- Product number
- MA5-14773 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (5A3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14773 targets CD14 in indirect ELISA, FACS and IHC applications and shows reactivity with Human samples. The MA5-14773 immunogen is purified recombinant fragment of human CD14 expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5A3
- Vial size
- 100 µL
- Concentration
- Conc. not determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.
Mori A, Murata S, Tashiro N, Tadokoro T, Okamoto S, Otsuka R, Wada H, Murata T, Takahashi T, Seino KI, Taniguchi H
Cells 2021 Feb 23;10(2)
Cells 2021 Feb 23;10(2)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node using CD14/LPS-R monoclonal antibody (Product # MA5-14773) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 hour at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD14 Monoclonal Antibody (5A3) (Product # MA5-14773) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 hour at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD14 Monoclonal Antibody (5A3) (Product # MA5-14773) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 hour at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD14 Monoclonal Antibody (5A3) (Product # MA5-14773) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Following antigen retrieval, the sections were blocked with 3% H2O2 for 1 hour at room temperature followed by 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD14 Monoclonal Antibody (5A3) (Product # MA5-14773) at 1:500 dilution in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Product # B40916). Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and externally deconvoluted.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human PBMC using CD14/LPS-R monoclonal antibody (Product # MA5-14773)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human PBMC using CD14/LPS-R monoclonal antibody (Product # MA5-14773)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 hiPSC-derived liver bud transplantations in NOG-HLA-A2Tg mice. ( a ) The image depicts the transplantation procedure. The yellow circle is the position where the liver bud was transplanted. ( b ) Hematoxylin and eosin staining. Top row shows low magnification, and bottom row depicts a zoomed-in image of the black frame border. Scale bar: 200 um. ( c ) Immunohistochemistry for human lymphocyte detection: mCD45 (green), hCD45 (red), CK8*18 (white), and DAPI (blue). Arrowheads represent hCD45 + cells. Scale bar: 50 um. ( d ) Count of the hCD45 + cells ratio. Error bar: SEM, Mann-Whitney U test, ns: no significance, HSC(+) 1w n = 18, and HSC( + ) 4w n = 31. ( e ) Immunohistochemistry for T-cell detection is shown: hCD3 (green), hCD14 (red), hNKp46 (white), and DAPI (blue). Arrowheads show hCD3 + cells. Scale bar: 50 um. ( f ) Immunohistochemistry for T-cell variation detection: hCD3 (green), hCD8 (red), hCD4 (white), and DAPI (blue). Arrowheads demonstrate hCD3 + hCD8 + cells, and arrows show hCD3 + hCD4 + cells. Scale bar: 50 um. ( g ) Count of the hCD8 + cell and hCD4 + cell ratio. Error bar: SEM, Mann-Whitney U test, ns, * p < 0.01 and ** p < 0.0001, HSC(+) 1w n = 15, and HSC( + ) 4w n = 22.
