MA5-14773

antibody from Invitrogen Antibodies

Targeting: CD14

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14773

Provider

Invitrogen Antibodies

Product name

CD14 Monoclonal Antibody (5A3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-14773 targets CD14 in indirect ELISA, FACS and IHC applications and shows reactivity with Human samples. The MA5-14773 immunogen is purified recombinant fragment of human CD14 expressed in E. Coli.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5A3

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.

Mori A, Murata S, Tashiro N, Tadokoro T, Okamoto S, Otsuka R, Wada H, Murata T, Takahashi T, Seino KI, Taniguchi H
Cells 2021 Feb 23;10(2)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lymph node using CD14/LPS-R monoclonal antibody (Product # MA5-14773) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of human PBMC using CD14/LPS-R monoclonal antibody (Product # MA5-14773)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 hiPSC-derived liver bud transplantations in NOG-HLA-A2Tg mice. ( a ) The image depicts the transplantation procedure. The yellow circle is the position where the liver bud was transplanted. ( b ) Hematoxylin and eosin staining. Top row shows low magnification, and bottom row depicts a zoomed-in image of the black frame border. Scale bar: 200 um. ( c ) Immunohistochemistry for human lymphocyte detection: mCD45 (green), hCD45 (red), CK8*18 (white), and DAPI (blue). Arrowheads represent hCD45 + cells. Scale bar: 50 um. ( d ) Count of the hCD45 + cells ratio. Error bar: SEM, Mann-Whitney U test, ns: no significance, HSC(+) 1w n = 18, and HSC( + ) 4w n = 31. ( e ) Immunohistochemistry for T-cell detection is shown: hCD3 (green), hCD14 (red), hNKp46 (white), and DAPI (blue). Arrowheads show hCD3 + cells. Scale bar: 50 um. ( f ) Immunohistochemistry for T-cell variation detection: hCD3 (green), hCD8 (red), hCD4 (white), and DAPI (blue). Arrowheads demonstrate hCD3 + hCD8 + cells, and arrows show hCD3 + hCD4 + cells. Scale bar: 50 um. ( g ) Count of the hCD8 + cell and hCD4 + cell ratio. Error bar: SEM, Mann-Whitney U test, ns, * p < 0.01 and ** p < 0.0001, HSC(+) 1w n = 15, and HSC( + ) 4w n = 22.