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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- SM3009B - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM3009B, RRID:AB_1610375
- Product name
- anti CD14
- Antibody type
- Monoclonal
- Antigen
- A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MEM-18
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
Submitted references Hepatic progenitor cells from adult human livers for cell transplantation.
CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression.
Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL.
CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.
Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.
Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
Weiss TS, Lichtenauer M, Kirchner S, Stock P, Aurich H, Christ B, Brockhoff G, Kunz-Schughart LA, Jauch KW, Schlitt HJ, Thasler WE
Gut 2008 Aug;57(8):1129-38
Gut 2008 Aug;57(8):1129-38
CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR
International immunology 2007 Nov;19(11):1271-9
International immunology 2007 Nov;19(11):1271-9
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ
International immunology 2007 May;19(5):675-84
International immunology 2007 May;19(5):675-84
The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
Biochemical and biophysical research communications 2005 Mar 4;328(1):173-6
Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression.
Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J
The Journal of experimental medicine 2002 Oct 21;196(8):1017-24
The Journal of experimental medicine 2002 Oct 21;196(8):1017-24
Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL.
Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2724-33
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2724-33
CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.
Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H
Infection and immunity 2001 Jun;69(6):3772-81
Infection and immunity 2001 Jun;69(6):3772-81
Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.
Bazil V, Baudys M, Hilgert I, Stefanová I, Low MG, Zbrozek J, Horejsí V
Molecular immunology 1989 Jul;26(7):657-62
Molecular immunology 1989 Jul;26(7):657-62
Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
Bazil V, Horejsí V, Baudys M, Kristofová H, Strominger JL, Kostka W, Hilgert I
European journal of immunology 1986 Dec;16(12):1583-9
European journal of immunology 1986 Dec;16(12):1583-9
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector; Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig. 2. Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC.
