Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB3831 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD14 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08571
- Isotype
- IgG
- Antibody clone number
- 134603
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references 1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-κB-dependent expression of CD55 in human monocytic THP-1 cells.
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome.
Izban MG, Nowicki BJ, Nowicki S
PloS one 2012;7(11):e49318
PloS one 2012;7(11):e49318
Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
Clinical and experimental immunology 2009 May;156(2):285-93
Clinical and experimental immunology 2009 May;156(2):285-93
Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome.
Srinivasan M, Kodumudi KN, Zunt SL
Clinical immunology (Orlando, Fla.) 2008 Jan;126(1):31-7
Clinical immunology (Orlando, Fla.) 2008 Jan;126(1):31-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD14 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 5 µg/mL Human CD14 Monoclonal Antibody (Catalog # MAB3831) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human CD14 (Catalog # 383-CD) (5 ng) was included. A specific band for CD14 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.