Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-214-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal G-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human G-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse G-CSF and recombinant canine G-CSF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of β-tubulin deactylase.
Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
Ho YK, Zhou LH, Tam KC, Too HP
Nucleic acids research 2017 Apr 7;45(6):e38
Nucleic acids research 2017 Apr 7;45(6):e38
Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
Rojahn A, Brusletto B, Øvstebø R, Haug KB, Kierulf P, Brandtzaeg P
Critical care medicine 2008 Sep;36(9):2583-9
Critical care medicine 2008 Sep;36(9):2583-9
Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
Blood 2005 Jan 15;105(2):830-7
Blood 2005 Jan 15;105(2):830-7
Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
Experimental hematology 2004 May;32(5):441-9
Experimental hematology 2004 May;32(5):441-9
Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
Infection and immunity 2002 Nov;70(11):6048-57
Infection and immunity 2002 Nov;70(11):6048-57
Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
Infection and immunity 2002 Nov;70(11):6048-57
Infection and immunity 2002 Nov;70(11):6048-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human G-CSF by Western Blot. Western blot shows 25 ng of Recombinant Human G-CSF (Catalog # 214-CS) and Recombinant Mouse G-CSF (Catalog # 414-CS). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G-CSF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA). The ND50 is typically 0.02-0.1 µg/mL.