AB-214-NA

antibody from Novus Biologicals

Targeting: CSF3 C17orf33, G-CSF, GCSF, MGC45931

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-214-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal G-CSF Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human G-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse G-CSF is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.

Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Recombinant Human and Mouse G-CSF by Western Blot. Western blot shows 25 ng of Recombinant Human G-CSF (Catalog # 214-CS) and Recombinant Mouse G-CSF (Catalog # 414-CS). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human G-CSF Polyclonal Antibody (Catalog # AB-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G-CSF at approximately 15-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Human G-CSF Polyclonal Antibody (Catalog # AB-214-NA). The ND₅₀ is typically 0.1-0.3 µg/mL.