Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-214-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal G-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human G-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse G-CSF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64
Blood 2000 Jun 15;95(12):3758-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human and Mouse G-CSF by Western Blot. Western blot shows 25 ng of Recombinant Human G-CSF (Catalog # 214-CS) and Recombinant Mouse G-CSF (Catalog # 414-CS). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human G-CSF Polyclonal Antibody (Catalog # AB-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G-CSF at approximately 15-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Human G-CSF Polyclonal Antibody (Catalog # AB-214-NA). The ND50 is typically 0.1-0.3 µg/mL.