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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- AHC2034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G-CSF Monoclonal Antibody (BVD13-3A5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- BVD13-3A5
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Versatile and on-demand biologics co-production in yeast.
Cao J, Perez-Pinera P, Lowenhaupt K, Wu MR, Purcell O, de la Fuente-Nunez C, Lu TK
Nature communications 2018 Jan 8;9(1):77
Nature communications 2018 Jan 8;9(1):77
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GCSF (BVD13 3A5) was performed using 70% confluent log phase K562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with G-CSF BVD13-3A5) Rat Monoclonal Antibody (Product # AHC2034) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
