AHC2034

antibody from Invitrogen Antibodies

Targeting: CSF3 C17orf33, G-CSF, GCSF, MGC45931

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

AHC2034

Provider

Invitrogen Antibodies

Product name

G-CSF Monoclonal Antibody (BVD13-3A5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

BVD13-3A5

Vial size

500 µg

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Versatile and on-demand biologics co-production in yeast.

Cao J, Perez-Pinera P, Lowenhaupt K, Wu MR, Purcell O, de la Fuente-Nunez C, Lu TK
Nature communications 2018 Jan 8;9(1):77

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-G-CSF Monoclonal Antibody (BVD13-3A5) (Product # AHC2034) and a 20k Da band corresponding to G-CSF was observed. Recombinant human G-CSF (1 µg lysate) was electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved protein was then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Fab2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GCSF (BVD13 3A5) was performed using 70% confluent log phase K562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with G-CSF BVD13-3A5) Rat Monoclonal Antibody (Product # AHC2034) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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