MAB214-100

antibody from R&D Systems

Targeting: CSF3 C17orf33, G-CSF, GCSF, MGC45931

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB214-100

Provider

R&D Systems

Product name

Human G-CSF Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Captures human G-CSF in sandwich ELISAs. In this format, no cross-reactivity or interference was observed with recombinant human (rh) GM-CSF, rmGM-CSF, rhM-CSF, or rmM-CSF. This antibody cross-reacts with recombinant mouse G-CSF at concentrations greater than 50 ng/mL.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NP_757373


Isotype

IgG

Antibody clone number

3316

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.

Ramachandran R, Morice AH, Compton SJ
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human G-CSF Monoclonal Antibody (Catalog # MAB214). The ND₅₀ is typically 0.01-0.03 µg/mL.