Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB214R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human G-CSF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human G-CSF in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_757373
- Isotype
- IgG
- Antibody clone number
- 3316R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by G-CSF and Neutralization by Human G-CSF Antibody. Recombinant Human G-CSF (Catalog # 214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human G-CSF Monoclonal Antibody (Catalog # MAB214R). The ND50 is typically 0.01-0.03 μg/mL.