MA5-23693

antibody from Invitrogen Antibodies

Targeting: CSF3 C17orf33, G-CSF, GCSF, MGC45931

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23693

Provider

Invitrogen Antibodies

Product name

G-CSF Monoclonal Antibody (3316)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

No cross-reactivity or interference was observed with recombinant human (rh) GM-CSF, rmGM-CSF, rhM-CSF, or rmM-CSF. This antibody cross-reacts with recombinant mouse G-CSF at concentrations greater than 50 ng/mL.

Antibody clone number

3316

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-G-CSF Monoclonal Antibody (3316) (Product # MA5-23693) and a band around 20 kDa corresponding to CSF3/GCSF was observed. 1 µg of purified recombinant Human CSF3 was electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).