Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-7351-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G-CSF Monoclonal Antibody (8F5CSF), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: G-CSF (Granulocyte colony-stimulating factor) is a growth factor that stimulates the proliferation, differentiation, and mobilization of hematopoetic stem cells. It is expressed by monocytes, macrophages, and bone marrow stromal cells, and can also be induced in fibroblasts by IL-17A. Unlike GM-CSF and IL-3, which can stimulate cells of multiple lineages, G-CSF activity is limited to neutrophilic granulocytes. G-CSF is essential to the maintenance of neutrophil counts during homeostasis, and low basal levels of the protein are detectable in the serum of healthy individuals. Circulating levels become elevated rapidly upon infection, as G-CSF is also important for the activation and mobilization of mature neutrophils during the innate immune response. G-CSF therapy is commonly used to treat neutropenia following bone marrow transplant or chemotherapy. Applications Reported: This 8F5CSF antibody has been reported for use in intracellular staining and flow cytometric analysis. Applications Tested: This 8F5CSF antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 8F5CSF
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Homeostatic regulation of blood neutrophil counts.
Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis.
Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization.
von Vietinghoff S, Ley K
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 15;181(8):5183-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 15;181(8):5183-8
Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis.
Panopoulos AD, Watowich SS
Cytokine 2008 Jun;42(3):277-88
Cytokine 2008 Jun;42(3):277-88
Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization.
Lieschke GJ, Grail D, Hodgson G, Metcalf D, Stanley E, Cheers C, Fowler KJ, Basu S, Zhan YF, Dunn AR
Blood 1994 Sep 15;84(6):1737-46
Blood 1994 Sep 15;84(6):1737-46
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells unstimulated (left) or stimulated overnight with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right) with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human G-CSF PE (right). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye