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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2141P - Provider product page
- Provider
- R&D Systems
- Product name
- Human G-CSF PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human G-CSF in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
NP_757373- Isotype
- IgG
- Antibody clone number
- 5008
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis.
Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N
Blood 2004 Feb 1;103(3):1089-98
Blood 2004 Feb 1;103(3):1089-98
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of G-CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human IL-beta (Catalog # 201-LB) were stained with Mouse Anti-Human G-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2141P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
