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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-22287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, NIH-3T3, JC, BCL-1.
- Concentration
- 0.36 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSMA3 Polyclonal Antibody (Product # PA5-22287). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. PSMA3 Polyclonal Antibody (Product # PA5-22287) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Proteasome 20S alpha 3 antibody detects PSMA3 protein by Western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract. B. 30 µg JC whole cell lysate/extract. C. 30 µg BCL-1 whole cell lysate/extract.12 % SDS-PAGE. Proteasome 20S alpha 3 antibody PSMA3 Polyclonal Antibody (Product # PA5-22287) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PSMA3 was performed on human H1 huES teratoma tissue. To expose target proteins, antigen retrieval was performed using a pressure cooker in 10mM citric acid (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 2.5% hydrogen peroxide. Tissue slide was successively blocked with Avidin D, biotin and protein blocking buffer. Tissues were probed with a PSMA3 polyclonal antibody (Product # PA5-22287) diluted 1:100 overnight at 4°C. After washing tissue slide was incubated with biotinylated anti-rabbit IgG at 37°C for 30 min. After washing tissue slide was incubated at 37°C for 30 min with an HRP kit to conjugate biotinylated anti-rabbit IgG with biotinylated HRP/Avidin. DAB staining for development was performed for 5 min and tissue imaged. Data courtesy of the Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 (Product # PA5-22287) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
