ABIN362248

antibody from antibodies-online

Targeting: NFKBIE IKBE

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362248

Provider

antibodies-online

Product name

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) (pSer22) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer22 (I-E-S (p) -L-R) derived from Human IkB-e. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pSer22

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Discovering the role of the major histocompatibility complex in the immune response.

McDevitt HO
Annual review of immunology 2000;18:1-17

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Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1.

Shirane M, Hatakeyama S, Hattori K, Nakayama K, Nakayama K
The Journal of biological chemistry 1999 Oct 1;274(40):28169-74

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Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity.

Chen ZJ, Parent L, Maniatis T
Cell 1996 Mar 22;84(6):853-62

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Control of I kappa B-alpha proteolysis by site-specific, signal-induced phosphorylation.

Brown K, Gerstberger S, Carlson L, Franzoso G, Siebenlist U
Science (New York, N.Y.) 1995 Mar 10;267(5203):1485-8