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Learnsc-7184
antibody from Santa Cruz Biotechnology
Targeting: CHUK
IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-7184 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7184, RRID:AB_649197
- Product name
- Anti-CHUK
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction
UV as an amplifier rather than inducer of NF-kappaB activity.
Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.
Daming Gao, Hiroyuki Inuzuka, Alan Tseng, Rebecca Y. Chin, Alex Toker, Wenyi Wei
Nature Cell Biology 2009 Mar;11(4):397-408
Nature Cell Biology 2009 Mar;11(4):397-408
UV as an amplifier rather than inducer of NF-kappaB activity.
O'Dea EL, Kearns JD, Hoffmann A
Molecular cell 2008 Jun 6;30(5):632-41
Molecular cell 2008 Jun 6;30(5):632-41
Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.
Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt-Supprian M, Rajewsky K, Brenner DA, Manns MP, Pasparakis M, Trautwein C
The Journal of clinical investigation 2005 Apr;115(4):849-59
The Journal of clinical investigation 2005 Apr;115(4):849-59
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 85
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
