PA5-36652
antibody from Invitrogen Antibodies
Targeting: CHUK
IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-36652 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IKK alpha (Thr23) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 85 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IKK alpha pThr23 using Phospho-IKK alpha pThr23 polyclonal antibody (Product # PA5-36652) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Raw264.7 cell lysate treated with TNF-alpha, Lane 3: PC12 cell lysate treated with LPS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IKK alpha (Thr23) in Lane 1: K562 whole cell lysate (40 µg), Lane 2: HepG2 whole cell lysate (40 µg), Lane 3: HEK293T whole cell lysate (40 µg), Lane 4: the Embryo tissue lysate of rat (10 µg), Lane 5: the Brain tissue lysate of mouse (40 µg). Samples were incubated with Phospho-IKK alpha (Thr23) polyclonal antibody (Product # PA5-36652) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-IKK alpha pThr23 using Phospho-IKK alpha pThr23 polyclonal antibody (Product # PA5-36652) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Raw264.7 cell lysate treated with TNF-alpha, Lane 3: PC12 cell lysate treated with LPS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-IKK alpha (Thr23) in paraffin-embedded human colorectal carcinoma tissue. Samples were incubated with Phospho-IKK alpha (Thr23) polyclonal antibody (Product # PA5-36652) at a dilution of 1:50.