ARG54650

antibody from Arigo

Targeting: CHUK IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG54650

Provider

Arigo

Product name

anti-IKK alpha antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide around 19 aa (C-terminus) of Human IKK alpha protein (NP_001269.3).

Description

Affinity purification with immunogen.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Storage

For continuous use, store undiluted antibody at 4°C for up to 3 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 30 µg of HeLa cell lysates 1) untreated or 2) treated with 5 ng/ml TNF-a for 5 min, stained with ARG54650 anti-IKK alpha antibody at 1:500 dilution.Data provided by customer.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: HeLa whole cell lysate stained with ARG54650 anti-IKK alpha antibody at 1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunocytochemistry: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 1µg/ml.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 10 µg/ml.