PA5-85942
antibody from Invitrogen Antibodies
Targeting: CHUK
IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-85942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK alpha in different lysates using a Polyclonal antibody (Product #PA5-85942) at a dilution of 1:500. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: Raji, Lane 4: NIH/3T3, Lane 5: PC12, Lane 6: Jurkat.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IKK alpha in N2A cells using a IKK alpha Polyclonal antibody (Product # PA5-85942) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IKK alpha of paraffin-embedded Human mammary gland tissue using a IKK-alpha Polyclonal antibody (Product #PA5-85942). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IKK alpha of paraffin-embedded Human gastric carcinoma tissue using a IKK-alpha Polyclonal antibody (Product #PA5-85942). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of IKK alpha in Hela cells using a IKK alpha Polyclonal Antibody (Product # PA5-85942) at a dilution of 1:100, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.