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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1716 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-23A/IL-23 P19 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-23 p19 in direct ELISA and Western blot. In direct ELISA, approximately 50% cross-reactivity with recombinant canine IL-23p19, less then 25% cross-reactivity with recombinant feline IL-23p19, less than 5% cross-reactivity with recombinant mouse IL-23p19, recombinant human IL-12p40, and recombinant rat IL-23p40 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes.
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
Olson BM, Jankowska-Gan E, Becker JT, Vignali DA, Burlingham WJ, McNeel DG
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature 2012 Apr 26;484(7395):514-8
Nature 2012 Apr 26;484(7395):514-8
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
The British journal of dermatology 2011 Jun;164(6):1228-34
The British journal of dermatology 2011 Jun;164(6):1228-34
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes.
Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4175-82
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4175-82
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Aliahmadi E, Gramlich R, Grützkau A, Hitzler M, Krüger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
European journal of immunology 2009 May;39(5):1221-30
European journal of immunology 2009 May;39(5):1221-30
IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
de Paus RA, van de Wetering D, van Dissel JT, van de Vosse E
Molecular immunology 2008 Sep;45(15):3889-95
Molecular immunology 2008 Sep;45(15):3889-95
Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer GA, van Seventer JM
Cytokine 2006 Sep;35(5-6):235-46
Cytokine 2006 Sep;35(5-6):235-46
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of human IL-23 p19 by Western Blot. Western blot shows lysates of a CHO cell line transfected with human IL-23. A PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-17 Secretion Induced by IL-23 and Neutralization by Human IL-23 Antibody. In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stim-ulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND50 is typically 0.2-0.8 µg/mL.
