AF6250

antibody from Novus Biologicals

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6250

Provider

Novus Biologicals

Product name

Sheep Polyclonal IL-23A/IL-23 P19 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and canine IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant feline IL-23 p19 is observed and approximately 40% cross-reactivity with recombinant mouse IL-23 p19 is observed.

Reactivity

Human, Canine

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Canine IL-23 p19 by Western Blot. Western blot shows lysates of human mature dendritic cells (LPS activated), CLL-1390 canine leukocytic round cell neoplasia cell line, and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Canine IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6250) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.