IC17161P

antibody from Novus Biologicals

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC17161P

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-23A/IL-23 P19 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-23 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), (A) treated with LPS, or (B) resting, were stained with Mouse Anti-Human IL-23 p19 PE-conjugated Monoclonal Antibody (Catalog # IC17161P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.