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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC18871P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-23 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-23 in flow cytometry.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
Q9EQ14 (Il-23 p19) & P43432 (IL
-12/IL-23 p40)- Isotype
- IgG
- Antibody clone number
- 320244
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-23 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line untreated (open histogram) or treated with LPS (filled histogram) was stained with Rat Anti-Mouse IL-23 PE-conjugated Monoclonal Antibody (Catalog # IC18871P, filled histogram) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
