Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-23613 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-23 p19 Monoclonal Antibody (727753), Alexa Fluor 488
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 727753
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometric analysis of human peripheral blood mononuclear cells (PBMCs) either (A) treated with LPS or (B) untreated, were stained with mouse Anti-human CD14 APC-conjugated Monoclonal Antibody and mouse Anti-human IL-23 p19 Alexa Fluor® 488-conjugated Monoclonal Antibody (Product # MA5-23613). Quadrant markers were set based on isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.
- Conjugate
- Green dye