Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations [0]
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- Product number
- 14-7129-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-12/IL-23 p40 Monoclonal Antibody (C8.6), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Reported: Unlabeled C8.6 has been reported for use as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and in immunblotting under nonreducing conditions. C8.6 has also been reported for use in cytokine neutralization. (Please use Functional Grade purified C8.6, cat. 16-7129, in functional assays.). Applications Tested: The unconjugated C8.6 antibody has been tested as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and nonreducing immunoblots at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- C8.6
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.
Immune activation and regulation in simian immunodeficiency virus-Plasmodium fragile-coinfected rhesus macaques.
Src-kinase inhibitors sensitize human cells of myeloid origin to Toll-like-receptor-induced interleukin 12 synthesis.
Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae.
Tsai CY, Liong KH, Gunalan MG, Li N, Lim DS, Fisher DA, MacAry PA, Leo YS, Wong SC, Puan KJ, Wong SB
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3890-900
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3890-900
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
Deng N, Weaver JM, Mosmann TR
PloS one 2014;9(5):e95986
PloS one 2014;9(5):e95986
Dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients.
Lopes DM, Fernandes JS, Cardoso TM, Bafica AM, Oliveira SC, Carvalho EM, Araujo MI, Cardoso LS
BioMed research international 2014;2014:743069
BioMed research international 2014;2014:743069
The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.
Lim DS, Yawata N, Selva KJ, Li N, Tsai CY, Yeong LH, Liong KH, Ooi EE, Chong MK, Ng ML, Leo YS, Yawata M, Wong SB
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):5065-75
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):5065-75
Immune activation and regulation in simian immunodeficiency virus-Plasmodium fragile-coinfected rhesus macaques.
Trott KA, Richardson A, Hudgens MA, Abel K
Journal of virology 2013 Sep;87(17):9523-37
Journal of virology 2013 Sep;87(17):9523-37
Src-kinase inhibitors sensitize human cells of myeloid origin to Toll-like-receptor-induced interleukin 12 synthesis.
Wölfl M, Schwinn S, Yoo YE, Reß ML, Braun M, Chopra M, Schreiber SC, Ayala VI, Ohlen C, Eyrich M, Beilhack A, Schlegel PG
Blood 2013 Aug 15;122(7):1203-13
Blood 2013 Aug 15;122(7):1203-13
Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae.
Olliver M, Hiew J, Mellroth P, Henriques-Normark B, Bergman P
Infection and immunity 2011 Oct;79(10):4210-7
Infection and immunity 2011 Oct;79(10):4210-7
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