16-5236-82

antibody from Invitrogen Antibodies
Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF
Provider product page for 16-5236-82
Blocking/Neutralizing
Functional assay
Other assay

Antibody data

Product number
16-5236-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-23 p19 Monoclonal Antibody (HNU2319), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The HNU2319 monoclonal antibody is specific to the p19 subunit of human IL-23, a heterodimeric cytokine made up of two covalently linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as the beta-glucan receptor Dectin-1. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. In contrast, the mouse IL-23 biological activities are more distinctly different than those of mouse IL-12 suggesting the signaling or response elements (receptors) differ between species. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to IL-23. Signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which drives the differentiation of CD4^+ T-cells towards the Th17 phenotype. The monoclonal antibody HNU2319 neutralizes the bioactivity of IL-23 without affecting IL-12. Applications Reported: The monoclonal antibody HNU2319 reacts with the p19 subunit of human IL-23 and inhibits its bioactivity. Applications Tested: The ND50 of HNU2319, as determined by the neutralization of mouse IL-17A induction in balb/c splenocytes by recombinant human IL-23, is 0.2-0.4 µg/mL in the presence of 2 ng/mL IL-23. This antibody is specific to the p19 subunit of IL-23, and will not affect the bioactivity of IL-12. The neutralization dose of this antibody will vary depending on cell type, assay method, and concentration of cytokine. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
HNU2319
Vial size
100 µg
Concentration
1 mg/mL
Storage
4°C
IL23A protein structure - 16-5236-82 shown in red.