Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations [0]
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- Product number
- 14-7238-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-23 p19 Antibody Cocktail, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The antibody eBio473P19 reacts with the p19 subunit of human IL-23. The eBio473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer. The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/mL with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/mL with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naive and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of naive CD4 cells with TGF beta1 and IL-6. Applications Reported: This eBio473P19 antibody has been reported for use in immunoblotting (WB), and ELISA. Applications Tested: The eBio473P19 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C8.6 antibody (13-7129) for detection and recombinant human IL-23 (14-8239) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2.5-8.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant IL-23 standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. TMB, rather than ABTS, should be used as the substrate.The antibody eBio473p19 also works in immunoblotting under both reducing and nonreducing conditions at 2 µg/mL. *eBio473P19 is a cocktail of the two clones, eBio11P19 and eBio25P19 at 0.5 mg/mL each. Please contact Tech Support for more information regarding the concentration. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBio473P19 (eBio11P19, eBio25P19)
- Vial size
- 500 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Human mast cells capture, store, and release bioactive, exogenous IL-17A.
Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.
A role for RUNX3 in inflammation-induced expression of IL23A in gastric epithelial cells.
Noordenbos T, Blijdorp I, Chen S, Stap J, Mul E, Cañete JD, Lubberts E, Yeremenko N, Baeten D
Journal of leukocyte biology 2016 Sep;100(3):453-62
Journal of leukocyte biology 2016 Sep;100(3):453-62
Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.
Cañete JD, Celis R, Yeremenko N, Sanmartí R, van Duivenvoorde L, Ramírez J, Blijdorp I, García-Herrero CM, Pablos JL, Baeten DL
Arthritis research & therapy 2015 Jul 9;17(1):173
Arthritis research & therapy 2015 Jul 9;17(1):173
A role for RUNX3 in inflammation-induced expression of IL23A in gastric epithelial cells.
Hor YT, Voon DC, Koo JK, Wang H, Lau WM, Ashktorab H, Chan SL, Ito Y
Cell reports 2014 Jul 10;8(1):50-8
Cell reports 2014 Jul 10;8(1):50-8
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