Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 50-7823-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-23 p19 Monoclonal Antibody (23dcdp), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells. Dendritic cells and macrophages produce high levels of p40 and low levels of IL-23 in response to TLR2, TLR4, and TLR8 ligands and agonists of the beta-glucan receptor, Dectin-1. This is due to the presence of monomer and dimer forms of p40, while only a small portion associates with p19 to form IL-23. Our studies suggest donor variability, expression kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40. Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of cultured human dendritic cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23dcdp
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit.
Abundant expression of the interleukin (IL)23 subunit p19, but low levels of bioactive IL23 in the rheumatoid synovium: differential expression and Toll-like receptor-(TLR) dependent regulation of the IL23 subunits, p19 and p40, in rheumatoid arthritis.
Kalim KW, Groettrup M
Molecular immunology 2013 Mar;53(3):274-82
Molecular immunology 2013 Mar;53(3):274-82
Abundant expression of the interleukin (IL)23 subunit p19, but low levels of bioactive IL23 in the rheumatoid synovium: differential expression and Toll-like receptor-(TLR) dependent regulation of the IL23 subunits, p19 and p40, in rheumatoid arthritis.
Brentano F, Ospelt C, Stanczyk J, Gay RE, Gay S, Kyburz D
Annals of the rheumatic diseases 2009 Jan;68(1):143-50
Annals of the rheumatic diseases 2009 Jan;68(1):143-50
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Intracellular staining of unstimulated (left) or LPS-stimulated and monensin-treated (right), in vitro-cultured human dendritic cells with Anti-Human IL-12/23 p40 PE (Product # 12-7235-42) and Anti-Human IL-23 p19 eFluor® 660. Cultures were fixed and permeabilized with the Fixation and Permeabilization Kit (Product # 88-8823-88). Total viable cells as determined by staining with the Fixable Viability Dye eFluor® 450 (Product # 65-0863-14) were used for analysis. Data from two donors are shown to illustrate variability in expression.