Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-77257 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77257, RRID:AB_11017985
- Product name
- Rabbit Polyclonal IL-23A/IL-23 P19 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation.
Li H, Yao Q, Mariscal AG, Wu X, Hülse J, Pedersen E, Helin K, Waisman A, Vinkel C, Thomsen SF, Avgustinova A, Benitah SA, Lovato P, Norsgaard H, Mortensen MS, Veng L, Rozell B, Brakebusch C
Nature communications 2018 Apr 12;9(1):1420
Nature communications 2018 Apr 12;9(1):1420
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein. Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane. Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues