PA5-20239

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Provider product page for PA5-20239

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [2]
Other assay [1]

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Product number: PA5-20239 - Provider product page
Provider: Invitrogen Antibodies
Product name: IL-23 p19 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is mouse pancreas tissue lysate. PA5-20239 can be used with blocking peptide PEP-0358.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4° C

IL23A protein structure - PA5-20239 shown in red.

Submitted references Primary alterations during the development of hidradenitis suppurativa.

Dajnoki Z, Somogyi O, Medgyesi B, Jenei A, Szabó L, Gáspár K, Hendrik Z, Gergely P, Imre D, Póliska S, Törőcsik D, Zouboulis CC, Prens EP, Kapitány A, Szegedi A
Journal of the European Academy of Dermatology and Venereology : JEADV 2022 Mar;36(3):462-471

Spontaneous pulmonary hypertension in genetic mouse models of natural killer cell deficiency.

Rätsep MT, Moore SD, Jafri S, Mitchell M, Brady HJM, Mandelboim O, Southwood M, Morrell NW, Colucci F, Ormiston ML
American journal of physiology. Lung cellular and molecular physiology 2018 Dec 1;315(6):L977-L990

IL23 and TGF-ß diminish macrophage associated metastasis in pancreatic carcinoma.

Hussain SM, Reed LF, Krasnick BA, Miranda-Carboni G, Fields RC, Bi Y, Elahi A, Ajidahun A, Dickson PV, Deneve JL, Hawkins WG, Shibata D, Glazer ES
Scientific reports 2018 Apr 11;8(1):5808

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of mouse pancreas tissue lysate using a IL-23 polyclonal antibody (Product # PA5-20239) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse Pancreas Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20239) (1: 0.25 µg/mL, 2: 0.5 µg/mL, 3: 1 µg/mL, and 4: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23 with IL-23 p19 Polyclonal Antibody (Product # PA5-20239) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using IL-23 p19 Polyclonal Antibody (Product # PA5-20239) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6. Increased IL-23 production and downstream cytokine expression in the Ncr1-Gfp and Nfil3 -/- mouse models of natural killer cell impairment and deficiency. Assessment of inflammatory gene expression in the lungs of Ncr1 +/+ , Ncr1 +/gfp , and Ncr1 gfp/gfp mice ( A ), normalized to the Rpl32 reference gene ( n = 4-11; 1-way ANOVA, Dunnett's posttest vs. Ncr1 +/+ controls). Representative immunofluorescent staining ( B ) of mouse lung tissue for IL-23 (magenta), macrophages (F4/80, yellow), and nuclei (DAPI, blue). Scale bar = 50 mum. Quantification of IL-23 + cells ( C and G ), F4/80 + macrophages ( D and H ), IL-23 + macrophages ( E and I ), and IL-23 + F4/80 - cells ( F and J ) in the lungs of both Ncr1-Gfp mice ( C - F ) ( n = 5-6; 1-way ANOVA, Dunnett's posttest vs. Ncr1 +/+ controls) and Nfil3 -/- mice ( G - J ) with pulmonary hypertension or WT littermate controls ( n = 6-13; Student's t -test). Expression of Th17-associated cytokines ( K ) in the lungs of Nfil3 -/- mice with pulmonary hypertension and WT littermate controls ( n = 5-13; Student's t -test). * P < 0.05 and ** P < 0.01. Means +- SE.