M121B

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Western blot (Recommended by provider)

ELISA (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M121B

Provider

Invitrogen Antibodies

Product name

IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit. The M121B IL12 antibody (biotinylated conjugate of clone C8.6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M122 (clone 20C2). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.06 - 0.3 µg/mL for detection with 50 µl/well. Biotinylated antibody M121B (clone C8.6) and antibody M122 (clone 20C2) have successfully been used in combination with recombinant IL12 p70 protein SIL12 in ELISA applications.

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

C8.6

Vial size

250 µg

Concentration

0.5 mg/mL

Storage

-20°C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of Human IL-12 Biotinylated Antibody (Product # M121B) was performed using the Thermo Scientific Human IL-12 (p70) ELISA Kit (Product # EHIL12) by loading 50 µL per well of Human IL-12 Biotinylated Antibody (Product # M121B) in duplicate at 0.03125 µg/mL dilution and 50 µL per well of Human IL-12 (p70) ELISA Standard (Product # SIL12) in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/mL across a pre-coated plate of Human IL-12 MAb (Product # M122) at 7 µg/mL dilution and incubated for 3 hours at room temperature. The plate was washed then incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:3,000 dilution for 30 minutes at room temperature followed by 100 µL per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 TLR ligation induces differential cytokine production by human blood DCs. The DCs were stimulated as indicated and cultured overnight at 37degC. IL-10 and IL-12p70 production was analyzed in supernatants of overnight cultures by standard sandwich ELISA ( n >= 6). Each symbol represents one donor (also across the subsets). Significance was determined by Kruskal-Wallis test followed by Dunn's testing comparing different conditions of the same subset ( ** P < .01; *** P < .001).

Conjugate

Biotin