M121B

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

M121B

Provider

Invitrogen Antibodies

Product name

IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.

Conjugate

Biotin

Antibody clone number

C8.6

Concentration

0.5 mg/mL

References

Submitted references

Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1.

Kristoff J, Palma ML, Garcia-Bates TM, Shen C, Sluis-Cremer N, Gupta P, Rinaldo CR, Mailliard RB
EBioMedicine 2019 May;43:295-306

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The Phosphoinositide Kinase PIKfyve Promotes Cathepsin-S-Mediated Major Histocompatibility Complex Class II Antigen Presentation.

Baranov MV, Bianchi F, Schirmacher A, van Aart MAC, Maassen S, Muntjewerff EM, Dingjan I, Ter Beest M, Verdoes M, Keyser SGL, Bertozzi CR, Diederichsen U, van den Bogaart G
iScience 2019 Jan 25;11:160-177

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A Comparative Study of the T Cell Stimulatory and Polarizing Capacity of Human Primary Blood Dendritic Cell Subsets.

Sittig SP, Bakdash G, Weiden J, Sköld AE, Tel J, Figdor CG, de Vries IJ, Schreibelt G
Mediators of inflammation 2016;2016:3605643

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High production of proinflammatory and Th1 cytokines by dendritic cells from patients with rheumatoid arthritis, and down regulation upon FcgammaR triggering.

Radstake TR, van Lent PL, Pesman GJ, Blom AB, Sweep FG, Rönnelid J, Adema GJ, Barrera P, van den Berg WB
Annals of the rheumatic diseases 2004 Jun;63(6):696-702

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Interleukin 6 and 12, alanine aminotransferase activity, and HCV viral load in children with chronic hepatitis C treated with interferon and ribavirin.

Kowala-Piaskowska A, Mozer-Lisewska I, Figlerowicz M, Machowska L, Słuzewski W
Inflammation 2004 Dec;28(6):319-25

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Altered maturation of peripheral blood dendritic cells in patients with breast cancer.

Della Bella S, Gennaro M, Vaccari M, Ferraris C, Nicola S, Riva A, Clerici M, Greco M, Villa ML
British journal of cancer 2003 Oct 20;89(8):1463-72

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Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

McDowell MA, Marovich M, Lira R, Braun M, Sacks D
Infection and immunity 2002 Aug;70(8):3994-4001

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Monocyte induction of IL-10 and down-regulation of IL-12 by iC3b deposited in ultraviolet-exposed human skin.

Yoshida Y, Kang K, Berger M, Chen G, Gilliam AC, Moser A, Wu L, Hammerberg C, Cooper KD
Journal of immunology (Baltimore, Md. : 1950) 1998 Dec 1;161(11):5873-9

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IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells.

Kaliński P, Hilkens CM, Snijders A, Snijdewint FG, Kapsenberg ML
Journal of immunology (Baltimore, Md. : 1950) 1997 Jul 1;159(1):28-35

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Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells.

Kremer IB, Hilkens CM, Sylva-Steenland RM, Koomen CW, Kapsenberg ML, Bos JD, Teunissen MB
Journal of immunology (Baltimore, Md. : 1950) 1996 Sep 1;157(5):1913-8

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Regulation of bioactive IL-12 production in lipopolysaccharide-stimulated human monocytes is determined by the expression of the p35 subunit.

Snijders A, Hilkens CM, van der Pouw Kraan TC, Engel M, Aarden LA, Kapsenberg ML
Journal of immunology (Baltimore, Md. : 1950) 1996 Feb 1;156(3):1207-12

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Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.

Manetti R, Parronchi P, Giudizi MG, Piccinni MP, Maggi E, Trinchieri G, Romagnani S
The Journal of experimental medicine 1993 Apr 1;177(4):1199-204

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of Human IL-12 Biotinylated Antibody (Product # M121B) was performed using the Thermo Scientific Human IL-12 (p70) ELISA Kit (Product # EHIL12) by loading 50 µL per well of Human IL-12 Biotinylated Antibody (Product # M121B) in duplicate at 0.03125 µg/mL dilution and 50 µL per well of Human IL-12 (p70) ELISA Standard (Product # SIL12) in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/mL across a pre-coated plate of Human IL-12 MAb (Product # M122) at 7 µg/mL dilution and incubated for 3 hours at room temperature. The plate was washed then incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:3,000 dilution for 30 minutes at room temperature followed by 100 µL per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 TLR ligation induces differential cytokine production by human blood DCs. The DCs were stimulated as indicated and cultured overnight at 37degC. IL-10 and IL-12p70 production was analyzed in supernatants of overnight cultures by standard sandwich ELISA ( n >= 6). Each symbol represents one donor (also across the subsets). Significance was determined by Kruskal-Wallis test followed by Dunn's testing comparing different conditions of the same subset ( ** P < .01; *** P < .001).

Conjugate

Biotin