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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC17161G - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-23 p19 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed.. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
Q9NPF7- Isotype
- IgG
- Antibody clone number
- 727753
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Role of interleukin-23 in monocyte-derived dendritic cells of HBV-related acute-on-chronic liver failure and its correlation with the severity of liver damage.
Bao S, Zheng J, Li N, Huang C, Chen M, Cheng Q, Li Q, Lu Q, Zhu M, Ling Q, Yu K, Chen S, Shi G
Clinics and research in hepatology and gastroenterology 2017 Mar;41(2):147-155
Clinics and research in hepatology and gastroenterology 2017 Mar;41(2):147-155
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-23 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with LPS or (B) untreated, were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and Mouse Anti-Human IL-23 p19 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC17161G). Quadrant markers were set based on isotype control (Catalog # IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
