IC17161P

antibody from R&D Systems

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC17161P

Provider

R&D Systems

Product name

Human IL-23 p19 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.

Reactivity

Human

Host

Mouse

Antigen sequence

Q9NPF7


Isotype

IgG

Antibody clone number

727753

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-23 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), (A) treated with LPS, or (B) resting, were stained with Mouse Anti-Human IL-23 p19 PE-conjugated Monoclonal Antibody (Catalog # IC17161P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.