R31599

antibody from NSJ Bioreagents

Targeting: JUP CTNNG, DP3, DPIII, PDGB, PKGB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31599

Provider

NSJ Bioreagents

Product name

Plakoglobin Antibody / Gamma Catenin

Antibody type

Polyclonal

Description

This highly specific Plakoglobin antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunocytochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Plakoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Plakoglobin antibody and Lane 1: rat brain; 2: (r) heart; 3: (r) thymus; 4: (r) RH35; 5: human HeLa; 6: (h) COLO320; 7: (h) HepG2; 8: mouse HepA; 9: (h) MCF-7. Expected/observed size ~86KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Plakoglobin antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) rat heart, 3) rat thymus, 4) rat RH35, 5) human HeLa, 6) human COLO-320, 7) human HepG2, 8) mouse HEPA1-6 and 9) human MCF7 cell lysate with Plakoglobin antibody. Expected molecular weight: ~86 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Plakoglobin antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human 293T, 2) MCF7, 3) T-47D and 4) rat heart tissue lysate with Plakoglobin antibody. Expected molecular weight: ~86 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human A431 cells with Plakoglobin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC staining of FFPE human MCF7 with Plakoglobin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Plakoglobin antibody testing of human intestine cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC(P) testing of Plakoglobin antibody and mouse intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC(P) staining of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC(F) staining of rat intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Plakoglobin antibody testing of human intestine cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC(P) testing of Plakoglobin antibody and mouse intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC(P) staining of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of frozen rat intestine tissue.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A431 cells with Plakoglobin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Plakoglobin antibody.