PAB7924

antibody from Abnova Corporation

Targeting: JUP CTNNG, DP3, DPIII, PDGB, PKGB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7924

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7924, RRID:AB_1709809

Product name

JUP (phospho Y550) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUP.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.

Miravet S, Piedra J, Castaño J, Raurell I, Francí C, Duñach M, García de Herreros A
Molecular and cellular biology 2003 Oct;23(20):7391-402

---

A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.

McCrea PD, Turck CW, Gumbiner B
Science (New York, N.Y.) 1991 Nov 29;254(5036):1359-61

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of JUP immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse JUP monoclonal antibody. The blots were probed with either JUP (phospho Y550) polyclonal antibody (Cat # PAB7924). The immunoprecipitated JUP was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies. In addition, the JUP (phospho Y550) polyclonal antibody (Cat # PAB7924) was used in the presence of phospho-JUP (Tyr-550) peptide (lane 3) or phospho-JUP (Tyr-644) peptide (lane 4).