Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB7924 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7924, RRID:AB_1709809
- Product name
- JUP (phospho Y550) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUP.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription.
A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.
Miravet S, Piedra J, Castaño J, Raurell I, Francí C, Duñach M, García de Herreros A
Molecular and cellular biology 2003 Oct;23(20):7391-402
Molecular and cellular biology 2003 Oct;23(20):7391-402
A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.
McCrea PD, Turck CW, Gumbiner B
Science (New York, N.Y.) 1991 Nov 29;254(5036):1359-61
Science (New York, N.Y.) 1991 Nov 29;254(5036):1359-61
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of JUP immunoprecipitated from lysates prepared from A-431 cells treated with pervanadate. The immunoprecipitation was performed using mouse JUP monoclonal antibody. The blots were probed with either JUP (phospho Y550) polyclonal antibody (Cat # PAB7924). The immunoprecipitated JUP was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibodies. In addition, the JUP (phospho Y550) polyclonal antibody (Cat # PAB7924) was used in the presence of phospho-JUP (Tyr-550) peptide (lane 3) or phospho-JUP (Tyr-644) peptide (lane 4).