MAB7045

antibody from Novus Biologicals

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7045

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD44 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CD44 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant CD44 from mouse, rat, or pig is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy.

Giampieri R, Piva F, Occhipinti G, Bittoni A, Righetti A, Pagliaretta S, Murrone A, Bianchi F, Amantini C, Giulietti M, Ricci G, Principato G, Santoni G, Berardi R, Cascinu S
PloS one 2019;14(5):e0215990

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SALL4 promotes gastric cancer progression through activating CD44 expression.

Yuan X, Zhang X, Zhang W, Liang W, Zhang P, Shi H, Zhang B, Shao M, Yan Y, Qian H, Xu W
Oncogenesis 2016 Nov 7;5(11):e268

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD44 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

CD44 in Human Tonsil. CD44 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of CD44 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44s Pan Specific Monoclonal Antibody (Catalog # MAB7045, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophy-cocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).