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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM1901b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AM1901b, RRID:AB_10818722
- Product name
- CD44 Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD44 antibody (Cat. #AM1901b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD44 antibody detected the CD44 protein (arrow).
- Primary Ab dilution
- 1:100~500
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD44 antibody (Cat. #AM1901b) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD44 antibody (Cat. #AM1901b) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- CD44 Antibody (Cat. #AM1901b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
