GTX131669

antibody from GeneTex

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX131669

Provider

GeneTex

Product name

CD44 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD44 antibody detects CD44 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX131669) at a dilution of 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX131669) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX131669) diluted at 1:1000.