AF6577
antibody from R&D Systems
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF6577 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat CD44 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat CD44 in direct ELISAs. Detects mouse and rat CD44 in Western blots. In direct ELISA, approximately 50% cross-reactivity with recombinant mouse is observed.
- Reactivity
- Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O70509
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat CD44 by Western Blot. Western blot shows lysates of rat brain tissue, rat lung tissue, NR8383 rat alveolar macrophage cell line, and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Rat CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6577) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD44 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat CD44 by Simple WesternTM. Simple Western lane view shows lysates of NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 169 kDa (as indicated) using 10 µg/mL of Sheep Anti-Rat CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6577) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.