MHCD4401

antibody from Invitrogen Antibodies

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD4401

Provider

Invitrogen Antibodies

Product name

CD44 Monoclonal Antibody (MEM-85), FITC

Antibody type

Monoclonal

Antigen

Other

Description

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

MEM-85

Vial size

500 µL

Storage

4° C, store in dark

References

Submitted references

Assessing stemness and proliferation properties of the newly established colon cancer 'stem' cell line, CSC480 and novel approaches to identify dormant cancer cells.

Alowaidi F, Hashimi SM, Alqurashi N, Alhulais R, Ivanovski S, Bellette B, Meedenyia A, Lam A, Wood S
Oncology reports 2018 Jun;39(6):2881-2891

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Loss of quiescence and self-renewal capacity of hematopoietic stem cell in an in vitro leukemic niche.

Vanegas NP, Vernot JP
Experimental hematology & oncology 2017;6:2

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Induction of intestinal stemness and tumorigenicity by aberrant internalization of commensal non-pathogenic E. coli.

Sahu U, Choudhury A, Parvez S, Biswas S, Kar S
Cell death & disease 2017 Mar 16;8(3):e2667

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Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment.

Vernot JP, Bonilla X, Rodriguez-Pardo V, Vanegas NP
International journal of molecular sciences 2017 Feb 14;18(2)

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Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.

Onzi GR, Ledur PF, Hainzenreder LD, Bertoni AP, Silva AO, Lenz G, Wink MR
Cytotherapy 2016 Jul;18(7):828-37

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Human dental pulp stem cells respond to cues from the rat retina and differentiate to express the retinal neuronal marker rhodopsin.

Bray AF, Cevallos RR, Gazarian K, Lamas M
Neuroscience 2014 Nov 7;280:142-55

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Bimodal tumor-targeting from microenvironment responsive hyaluronan layer-by-layer (LbL) nanoparticles.

Dreaden EC, Morton SW, Shopsowitz KE, Choi JH, Deng ZJ, Cho NJ, Hammond PT
ACS nano 2014 Aug 26;8(8):8374-82

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Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection.

Avanzi S, Leoni V, Rotola A, Alviano F, Solimando L, Lanzoni G, Bonsi L, Di Luca D, Marchionni C, Alvisi G, Ripalti A
PloS one 2013;8(8):e71412

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Induction of tumorigenic stem cell-like properties in infected host cell. ( a ) Immunofluorescent staining of stem cell (SC) marker CD44. Scale bar=2 mu m. ( b ) FACS analysis of CD44 expression. =iMFI. ( c ) Protein levels of SC markers CD44 and CD133. ( d ) Sphere forming ability, showing (i) morphology, (ii) quantitative number and (iii) arbitrary size of spheroids. Scale bar=100 mu m. ( e ) mRNA levels of multipotency transcription factors and SC markers in adherent cells. ( f ) Self-renewing ability of microbe-induced SCs as measured by primary, secondary and tertiary spheroid formation. ( g ) Protein levels of pNF- kappa B and beta -catenin in spheroids. ( h ) mRNA levels of multipotency transcription factors and SC markers in spheroids. ( i ) Protein levels of SC markers in tumorspheres. ( j ) Tumorigenic ability of CD44 high and CD44 low cells of infected culture, showing (i) morphology and (ii) quantitative size. Scale bar=500 mu m. Data=Mean+-S.D. ( d , e , h ) and Mean+-S.E.M. ( j ) for three experiments; * P

Conjugate

Green dye