14-0441-85

antibody from Invitrogen Antibodies
Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Provider product page for 14-0441-85
Western blot
Immunocytochemistry
Immunoprecipitation
Immunohistochemistry
Flow cytometry
Other assay

Antibody data

Product number
14-0441-85 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD44 Monoclonal Antibody (IM7), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component. Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining and immunoblotting (non-reducing conditions). It has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified IM7, Product # 16-0441, in functional assays). Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
IM7
Vial size
500 µg
Concentration
0.5 mg/mL
Storage
4° C
CD44 protein structure - 14-0441-85 shown in red.