83-0441-42

antibody from Invitrogen Antibodies
Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Provider product page for 83-0441-42

Antibody data

Product number
83-0441-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD44 Monoclonal Antibody (IM7), eVolve 605, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Notice: The eVolve™ line of antibody conjugates will be discontinued at the end of February, 2018 and replaced with our improved Invitrogen™ eBioscience™ Super Bright conjugated antibodies for flow cytometry. Individual eVovle catalog numbers will become unavailable as inventory depletes. This item has a corresponding Super Bright 600 format available – please see Product # 63-0458-42. We will continue to offer a selection of Qdot conjugated antibodies. Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets. Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
IM7
Vial size
100 Tests
Concentration
5 µl/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD44 protein structure - 83-0441-42 shown in red.