BMS113FI
antibody from Invitrogen Antibodies
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- BMS113FI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD44std Monoclonal Antibody (SFF-2), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: BMS113 specifically recognizes human standard CD44, but cannot be used to distinguish between splice variants (exon v3-v10). CD44 is a cell membrane associated, polymorphic glycoprotein with apparent molecular weights ranging from 85 kDa to 250 kDa. CD44 isoforms participate in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastases formation and inflammation. Three isoform categories of the CD44 molecule have been identified: 1) an 80-90 kDa isoform, the so-called standard form named CD44std, which is widely distributed on several hematopoietic and nonhemato-poietic cells including all subsets of leukocytes, monocytes, erythrocytes, many types of epithelium, mesenchymal elements like fibroblasts, smooth muscle cells and glial cells of the central nervous system, 2) a medium size category of 110-160 kDa which is weakly expressed on epithelial cells and highly expressed in some carcinomas and 3) a category which includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate. These bigger isoforms of CD44 arise by alternative splicing of one or more "variant" exons (v2-v10) into the extracellular part of the 90 kDa constant form molecule. Compared to the standard CD44, all larger isoforms are expressed in a much more restricted fashion, only in a few normal tissues or on the surface of certain tumor cells. Some splice variants of
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- SFF-2
- Vial size
- 100 Tests
- Concentration
- 10 µL/Test
- Storage
- 4° C, store in dark
Submitted references Isoform switch of CD44 induces different chemotactic and tumorigenic ability in gallbladder cancer.
CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
Miwa T, Nagata T, Kojima H, Sekine S, Okumura T
International journal of oncology 2017 Sep;51(3):771-780
International journal of oncology 2017 Sep;51(3):771-780
CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
Gassenmaier M, Chen D, Buchner A, Henkel L, Schiemann M, Mack B, Schendel DJ, Zimmermann W, Pohla H
Stem cells (Dayton, Ohio) 2013 Aug;31(8):1467-76
Stem cells (Dayton, Ohio) 2013 Aug;31(8):1467-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD41 APC and CD44std FITC. As expected based on known relative expression patterns, CD44std clone SFF-2 stains lymphocytes (pink), monocytes (orange), granulocytes (blue) and a small subset of CD45 negative cells (green).
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 (A) Histogram of cell surface CD44 expression in the human gallbladder cancer cell line NOZ. (B) Double-staining analysis of CD44std and CD44v9. CD44std + -CD44v9 - (CD44s, orange) cells and CD44std - -CD44v9 + (CD44v, blue) cells were sorted. (C) Morphology of sorted NOZ-CD44v and CD44s cells cultured under normal conditions. (D) Reverse transcription-PCR analysis of mRNAs encoding CD44s, CD44v9, and CD44 (as well as pan-CD44) expressed by the sorted NOZ-CD44v and CD44s cells ( * p
- Conjugate
- Green dye